FastQCFastQC Report
Tue 28 Jun 2022
EGAF00006207023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006207023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260975
Sequences flagged as poor quality0
Sequence length101
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7825029.983714915221764No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCTAGTGTGTAGATC56822.1772200402337387Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCTAGTGTGTATATC15300.5862630520164767Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15250.5843471596896254No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCTAGTGTGTAGATA9600.36785132675543636Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT7310.28010345818564997No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCTAGTGTGTATATA6510.24944918095603028Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG5590.21419676214196764No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCTAGTGTGTAGAT5510.21113133441900564Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCCGAGTGTGTAGATC5250.20116869431937925Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAACAGTGTCTCCTAGTGTGTAGATC3800.14560781684069354Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG3490.13372928441421594No Hit
AGATCGGAAGAGCGTCGTGTAGGGTAAGAGTGTCTCCTAGTGTGTAGATC3040.11648625347255484Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGNGGGGGGGGGGGGGGGGGGGG2740.10499089951144745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)