FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006207025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006207025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383961
Sequences flagged as poor quality0
Sequence length101
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7295519.000627667914188No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCCTATCGTGTAGATC103562.697148929188121Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCCTATCGTGTAGATA15870.413323228140358Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCCTATCGTGTATATC13160.34274314318381294Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAACAGTGTAGCCTATCGTGTAGATC12410.32320990933975063Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGTAAGAGTGTAGCCTATCGTGTAGATC7790.20288518886032694Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCCAATCGTGTAGATC7240.1885608173746813Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCCTATCGTGTATATA6500.16928802664853984Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATCCTATCGTGTAGATC5660.14741080474319007Illumina Single End PCR Primer 1 (97% over 34bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCCTATAGTGTAGATC5000.13022155896041523Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT4470.11641807371061123No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG4030.1049585765220947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)