FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006207031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006207031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452828
Sequences flagged as poor quality0
Sequence length101
%GC75

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21360047.17022798943528No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCATGTGTAGATC20490.4524896870334873Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT17270.38138101000821506No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG11320.24998454159195102No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG6430.14199651964984497No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4660.10290883072601519No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCATGTGTAGATA4530.10003798351692034Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)