FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006207039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006207039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217563
Sequences flagged as poor quality0
Sequence length100
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6021527.67704067327625No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGTACCAGTGTAGATC20920.9615605594701305Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT6520.29968331012166594No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGTACCACTGTAGATC4610.2118926471872515Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG4170.1916686201238262No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGTACCAGTGTAGAT3860.17741987378368565Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGTACCAGTGTAGATA3730.17144459306040088Illumina Single End PCR Primer 1 (96% over 33bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3610.16592894931583035No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGTACCAGTGTATATC3440.15811512067768876Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG2810.12915799101869344No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAACAGTGTCTGTACCAGTGTAGATC2710.12456162123155132Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)