FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006207043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006207043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451384
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG414829.189957995852755No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCCTATCGTGTAGATC31570.6994044981656417Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT8350.1849866189319958No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG6040.13381068004182692No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCCTATCGTGTAGATA6020.1333675983198341Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGTAAGAGTGTAGCCTATCGTGTAGATC5220.11564432944012194Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)