FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006207047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006207047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506804
Sequences flagged as poor quality0
Sequence length101
%GC78

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26840052.959329444913614No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCAAGTGTAGATC15650.3087978784697832Illumina Single End PCR Primer 1 (97% over 34bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCAACTGTAGATC8610.16988816189296058Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT8140.16061435979195113No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8030.15844389547043827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)