FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006207059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006207059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549276
Sequences flagged as poor quality0
Sequence length101
%GC64

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14798526.94182888019866No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTGCTTGTGTAGATC130822.3816806122969143Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT24020.43730292239238566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG18600.3386275752080921No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTGCTTGTGTAGAT13260.24140869071286566Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG10880.19807892571312052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)