FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006207061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006207061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477329
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5651711.840261119688936No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACGGTTGTGTAGATC14650.3069161940715942Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACCGTTGTGTAGATC7670.16068581628185172Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT7010.1468588751154864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)