FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006207069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006207069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272227
Sequences flagged as poor quality0
Sequence length100
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6549724.059700176690775No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACCGGTTAGTGTAGATC10430.3831361327127728Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT9990.3669731510834708No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG5290.19432312004319927No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACCGGTTAGTGTAGATA3670.13481396040804183Illumina Single End PCR Primer 1 (97% over 34bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3640.13371193893331668No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG3410.12526310762709061No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)