FastQCFastQC Report
Thu 23 Jun 2022
EGAF00006207079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006207079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315112
Sequences flagged as poor quality0
Sequence length101
%GC74

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13977444.35692706085455No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGTGCATGTGTAGATC41541.3182614435502298Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGNGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26650.8457310416613776No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGTGCATGTGTATATC17270.5480591027951967Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT14060.4461905608164716No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGTGCATGTGTAGATA13270.42112010967529Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGTGCATGTGTATATA13230.419850719744091Illumina Single End PCR Primer 1 (96% over 33bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGTGCATGTGTAGAT10650.33797506918175124Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG8680.2754576150701972No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGTGCATGTGTATAT5510.1748584630226713Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG5380.17073294574627432No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5070.1608951737794816No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGNGGGGGGGGGGGGGGGGGGGG4780.15169209677828835No Hit
AGATCGGAAGAGCGTCGTGTAGGGTAAGAGTGTTCGTGCATGTGTAGATC3610.11456244129071569Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGTGCAAGTGTAGATC3330.10567671177232221Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)