FastQCFastQC Report
Sat 11 May 2024
EGAF00006209167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006209167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43240467
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGC652799315.096953046321168TruSeq Adapter, Index 19 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATCTCGTATGCC705460.16314809920993684TruSeq Adapter, Index 19 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGCC688540.15923509799281307TruSeq Adapter, Index 19 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)