FastQCFastQC Report
Sun 7 Aug 2022
EGAF00006209264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006209264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4071068
Sequences flagged as poor quality0
Sequence length35-75
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACAC504611.2395027545597372No Hit
NACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACAC273870.6727227351643352No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG247720.608488976357064No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT172150.422862010656663No Hit
NTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG134960.3315100607506433No Hit
NGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT90820.22308642351343677No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACAC45160.11092912228437353No Hit
CGCCATCTCCGCCATCTCCGCCATCTCCGCCATCTCCGCCATCTCCGCCATCTCCGCCATCTCCGCCATCTCCGC44520.10935705323517073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)