FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006209348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006209348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298006
Sequences flagged as poor quality0
Sequence length35-75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT44611413.526779514652187No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACAC2312247.011024237069307No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1563744.741471058572968No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACAC753572.2849261038336497No Hit
CTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG53870.16334112187788621No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47280.14335935107455838No Hit
CACACATACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACAC40640.12322597351247996No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG38160.11570627827845069No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACCC38110.11555467151970009No Hit
CACACACGCACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACAC35940.10897493818992446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)