FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006210070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006210070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65265732
Sequences flagged as poor quality0
Sequence length151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT12281001.8816919114612856Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG2534410.3883216999695951Illumina Single End PCR Primer 1 (97% over 49bp)
ATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT856640.13125417791989216Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCGT746950.11444750209803822Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG688720.10552551528878891Illumina Single End PCR Primer 1 (97% over 49bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG664830.10186509514671498Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)