FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006210072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006210072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65339797
Sequences flagged as poor quality0
Sequence length151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT12321931.8858231224685318Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG2376590.3637277905837387Illumina Single End PCR Primer 1 (97% over 49bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCGT802410.12280570752308889Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT798660.12223178471154417Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG707910.10834285267216243Illumina Single End PCR Primer 1 (97% over 49bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG660350.10106398096094484Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)