FastQCFastQC Report
Thu 6 Oct 2022
EGAF00006210078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006210078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61152707
Sequences flagged as poor quality0
Sequence length151
%GC39

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT9096721.4875416717039198Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG2061480.337103637946886Illumina Single End PCR Primer 1 (97% over 49bp)
ATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT712230.11646745253648379Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCGT665160.10877032802489021Illumina Single End PCR Primer 1 (98% over 50bp)
ATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGG625650.1023094529568413Illumina Single End PCR Primer 1 (97% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)