FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006210373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006210373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127639
Sequences flagged as poor quality0
Sequence length28-29203
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT103328.094704596557477No Hit
GGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTC69715.46149687791349No Hit
TGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATT33522.626156582235837No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG26522.0777348616018614No Hit
CTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19201.5042424337389042No Hit
TACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT18401.44156566566645No Hit
ATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACG12520.9808914203339104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7980.6252007615227321No Hit
TCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACG7260.5687916702575232No Hit
TATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAC5230.4097493712736703No Hit
CTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCG4930.3862455832464999No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.33218687078400805No Hit
GGTTTCGCTGGATAGTAGGTAGGGACGGTGGGAATCTCGTTCATCCATTC3340.2616755067024969No Hit
ACTTGCCTGTCACTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.2569747490970628No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAG2660.2084002538409107No Hit
TCTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.19508144062551414No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTCTTTTTTTTTTT2460.1927310618227971No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTCTTTTTTTTTTTT2300.18019570820830624No Hit
TCTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCC2080.1629595969883813No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTCTTTTTTTTTT1980.15512500097932452No Hit
TTTCTGTTGGTGCTGATATTGCTGGGGGGCCGCCGGTGAAATACCACTAC1710.13397159175487117No Hit
ACTTGCCTGTCAGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.13318813215396547No Hit
ACTTGCCTGTCAACTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.13005429375034275No Hit
ATTCGTACTGAAAATCAAGATCAAGCGAGCTTTGCCCTTCTGCTCCACGG1550.12143623814038029No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTCTTTTTTTTTTTTT1450.11360164213132351No Hit
TGGTTTCGCTGGATAGTAGGTAGGGACGGTGGGAATCTCGTTCATCCATT1430.11203472292951214No Hit
ACTTGCCTGTCCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.10733396532407807No Hit
TGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.1057670461222667No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACT1330.10420012692045535No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTCTTTTTTTTT1310.10263320771864398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)