FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006210374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006210374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328819
Sequences flagged as poor quality0
Sequence length1-25299
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT220456.704296284582095No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG143494.3637989288940116No Hit
GGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTC87812.6704661226997226No Hit
CTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43021.3083185582341652No Hit
TCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACG34271.0422147138699405No Hit
TGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATT33861.0297458480197312No Hit
TACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT32620.9920351317898297No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAG20900.6356080396814053No Hit
ATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACG20380.6197938683591885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20180.6137114947737204No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.3512570745607766No Hit
CTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCG8530.2594132334202099No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGA7000.21288307549137978No Hit
TATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAC6850.20832129530227875No Hit
TCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACA5280.160574662656355No Hit
TCTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.15905406925998802No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACT5100.1551005264294338No Hit
TTTCTGTTGGTGCTGATATTGCTGGGGGGCCGCCGGTGAAATACCACTAC5100.1551005264294338No Hit
ACTTGCCTGTCACTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT4950.15053874624033284No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTCTTTTTTTTTTT4770.14506461001341164No Hit
CTACGAATGGTTTAGCGCCGGGTTCCCCACGAACGTGCGGTGCGTGACGG4610.14019871114503724No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTCTTTTTTTTTT4300.13077103208756186No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTCTTTTTTTTTTTT3910.11891040359589926No Hit
ACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCC3750.11404450472752488No Hit
CGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGA3720.11313214868970468No Hit
GGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCA3640.11069919925551747No Hit
TGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.10644153774568989No Hit
TTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.10583330038714309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)