FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006210375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006210375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166816
Sequences flagged as poor quality0
Sequence length1-17469
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT65403.920487243429887No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG23361.4003452906196048No Hit
CTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15700.9411567235756763No Hit
TACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.5497074621139458No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8800.5275273355073854No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCA6850.41063207366199883No Hit
GGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTC5590.3350997506234414No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.2949357375791291No Hit
GGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC4450.2667609821599846No Hit
TCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACG4300.25776903894110875No Hit
TGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC3440.20621523115288704No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT3360.20141952810281988No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC3110.18643295607136007No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC2960.17744101285248418No Hit
TTTCTGTTGGTGCTGATATTGCTGGGCTCCTACTTGGATAACTGTGGTAA2720.16305390370228276No Hit
GGGTGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC2190.13128237099558795No Hit
GGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAAT2190.13128237099558795No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTCTTTTTTTTTTT2010.12049203913293688No Hit
TCTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.11629579896412816No Hit
TGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATT1860.11150009591406099No Hit
TTTCTGTTGGTGCTGATATTGCTGGGCGCGACCTCAGATCAGACGTGGCG1830.10970170727028583No Hit
GTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTA1820.10910224438902742No Hit
TTTCTGTTGGTGCTGATATTGCTGGGGGCTTTGGTGACTCTAGATAACCT1780.10670439286399386No Hit
ACTTGCCTGTCACTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.10610492998273546No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT1770.10610492998273546No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT1680.10070976405140994No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTCTTTTTTTTTT1680.10070976405140994No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAG1680.10070976405140994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)