FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006210376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006210376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392128
Sequences flagged as poor quality0
Sequence length1-37848
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT282777.211165741798596No Hit
GGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTC169154.313642484086829No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG130043.31626407703607No Hit
TGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATT77901.9865962134813124No Hit
CTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54201.3822017300473315No Hit
TACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT42881.0935204831075567No Hit
TCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACG30300.7727068712257222No Hit
ATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACG28120.7171127795005713No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21130.5388546597029541No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13390.34147013220173006No Hit
CTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCG12680.32336379957564876No Hit
TATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAC11660.2973518850987433No Hit
TTTCTGTTGGTGCTGATATTGCTGGGGGGCCGCCGGTGAAATACCACTAC9450.24099273706544802No Hit
ACTTGCCTGTCGCTCTATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7230.18437857026277132No Hit
CTACGAATGGTTTAGCGCCGGGTTCCCCACGAACGTGCGGTGCGTGACGG6450.1644871062510201No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTCTTTTTTTTTTT6330.16142688101844296No Hit
TCTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.16117186224906152No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTCTTTTTTTTTTTT6020.15352129916761872No Hit
ACTTGCCTGTCGCTCTATCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT5970.1522462053207116No Hit
GGTTTCGCTGGATAGTAGGTAGGGACGGTGGGAATCTCGTTCATCCATTC5400.1377101354659703No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACT5250.1338848539252489No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTCTTTTTTTTTT5240.13362983515586746No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAG5050.12878447853762037No Hit
TGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.12317406561122898No Hit
ACTTGCCTGTCGCTCTGTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.11909376530112616No Hit
TCTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCC4600.11730863391545616No Hit
GGTTTCGCTGGATAGTAGGTAGGGACAGGGGGAATCTCGTTCATCCATTC4200.10710788314019912No Hit
ACTTGCCTGTCACTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.10685286437081769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)