FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006210377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006210377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422740
Sequences flagged as poor quality0
Sequence length1-37852
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT4528410.712021573544023No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG100142.368831906136159No Hit
GGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTC63201.495008752424658No Hit
TACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT58971.3949472489000332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40270.9525949756351422No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29930.7080001892416142No Hit
TGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATT19720.46648057907933954No Hit
TCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACG18550.4388039929980603No Hit
ATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACG14980.3543549226474902No Hit
ACTTGCCTGTCACTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.33543076122439325No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAG9530.22543407295264228No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTCTTTTTTTTTTT9420.22283200075696646No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTCTTTTTTTTTTTT8720.20627335951175665No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTCTTTTTTTTTT7940.18782230212423712No Hit
CTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.17765056535932253No Hit
TTTCTGTTGGTGCTGATATTGCTGGGGGGCCGCCGGTGAAATACCACTAC7260.17173676491460474No Hit
CTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCG6990.16534986043430952No Hit
ACTTGCCTGTCCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT6660.15754364384728203No Hit
ACTTGCCTGTCAGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.15446846761602875No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTCTTTTTTTTT6530.15446846761602875No Hit
TTTCTGTTGGTGCTGATATTGCTGGGGTTGTTGCCATGGTAATCCTGCTC6020.14240431470880446No Hit
ACTTGCCTGTCAACTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.1409850026020722No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTCTTTTTTTTTTTTT5470.1293939537304253No Hit
TTTCTGTTGGTGCTGATATTGCTGGGTAGTAGGTAGGGACAGTGGGAATC5130.12135118512560912No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTCGTCACTACCTC4770.1128353124852155No Hit
ACTTGCCTGTCGCTCTATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.11188910441406064No Hit
ACTTGCCTGTCATCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.10928703221838483No Hit
CTACGAATGGTTTAGCGCCGGGTTCCCCACGAACGTGCGGTGCGTGACGG4310.10195391966693476No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTCGTCACTACCT4280.10124426361356864No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTT4280.10124426361356864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)