FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006210378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006210378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369850
Sequences flagged as poor quality0
Sequence length28-27654
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT286277.74016493172908No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG68411.8496687846424227No Hit
GGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTC60331.6312018385832092No Hit
TACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT36970.9995944301743951No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCA24210.6545896985264297No Hit
TGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATT19940.53913748817088No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18850.5096660808435852No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17910.48425037177234015No Hit
TCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACG15620.42233337839664725No Hit
TGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC14100.3812356360686765No Hit
ATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACG11700.3163444639718805No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTCTTTTTTTTTTT8930.24144923617682845No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTCTTTTTTTTTTTT8160.22062998512910637No Hit
ACTTGCCTGTCACTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT7730.20900365012843045No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTCTTTTTTTTTT7490.20251453291875082No Hit
CTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCG6240.1687170474516696No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTCTTTTTTTTT6150.16628362849803974No Hit
GGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC5400.146005137217791No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC5180.1400567797755847No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAG4970.13437880221711504No Hit
CTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.13167500337974855No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTCTTTTTTTTTTTTT4760.1287008246586454No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTCTTTTTTTT4510.12194132756522914No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT3820.10328511558740029No Hit
TATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAC3760.10166283628498039No Hit
ACTTGCCTGTCAGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.1011220765175071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)