FastQCFastQC Report
Fri 1 Jul 2022
EGAF00006210380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006210380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465981
Sequences flagged as poor quality0
Sequence length1-23389
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT310356.660142795521706No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG89991.9311946195231138No Hit
GGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTC74181.5919103997802486No Hit
TACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT35930.7710614810475106No Hit
TGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATT28930.6208407638938068No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28430.6101107126685423No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22830.4899341389455793No Hit
TCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACG22380.4802770928428412No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCA15480.33220238593419044No Hit
ATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACG14360.30816707118959785No Hit
TGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC13440.2884237769351111No Hit
CTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCG9070.19464312922629892No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTCTTTTTTTTTTT7380.15837555608490475No Hit
ACTTGCCTGTCGCTCTATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7340.1575171519868836No Hit
GTCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGG6910.14828930793315606No Hit
ACTTGCCTGTCGCTCTATCTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.13605704953635447No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTCTTTTTTTTTTTT6280.13476944338932273No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTCTTTTTTTTTT6040.12961901880119575No Hit
TTTCTGTTGGTGCTGATATTGCTGGGCGCGACCTCAGATCAGACGTGGCG5920.12704380650713226No Hit
CTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.10772971430165607No Hit
ACTTGCCTGTCGCTCTGTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.10687131020363493No Hit
TATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAC4970.10665670917912962No Hit
ACTTGCCTGTCGCTCTATCTTCTTTTTTTTTTTTTTTTTTCTTTTTTTTT4710.10107708254199206No Hit
ACTTGCCTGTCACTCTATCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.10000407741946561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)