FastQCFastQC Report
Tue 6 Sep 2022
EGAF00006214276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006214276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97323908
Sequences flagged as poor quality0
Sequence length98
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG9544620.9807066111648537Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8194450.8419770813149016Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC2823000.290062334940352Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCA2658780.27318878317134576Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGC2304140.2367496381259166Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1136500.11677500660988666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGC1112760.11433572930507475Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG1031890.1060263630186326Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)