FastQCFastQC Report
Wed 14 Sep 2022
EGAF00006214298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006214298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120663806
Sequences flagged as poor quality0
Sequence length98
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG18940661.569705169087738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC15749411.3052306670983012Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCA5404650.4479097899497717Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC5289830.43839409474619095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGC4504700.37332653007812466Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGC2123440.17597986259442205Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG1929130.15987644215366453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCG1767660.1464946331959726Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGGG1729670.143346216014436No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGCACAAATGTATGAACAG1588910.1316807460888479Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGCGCAGG1496150.12399327102279535No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1325690.10986641677786957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCGCAGG1302390.10793543177313669Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCACGC1300910.10781277693163434Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)