FastQCFastQC Report
Wed 14 Sep 2022
EGAF00006214535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006214535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405394781
Sequences flagged as poor quality0
Sequence length90
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT53113621.3101702954582437Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT23125530.5704446895679202Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT21975160.5420681525744655Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA18148610.4476774455564587Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA15233070.3757589074635867Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTC14830650.3658322873179761Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCG11993000.29583508624399385Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGGCGGCGACGACCCATTCG9500950.23436290858416353Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA9474340.23370651138204956Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAAC7003080.17274716716197686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA6992480.17248569364290853Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT6513890.16068016425697398No Hit
GAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAAC6386000.15752546158210162No Hit
ATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAA6259180.15439715293226727No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTC6258570.1543821058712643Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGA6133010.1512848780359607Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC5954070.14687090902633007No Hit
CTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCG5653450.1394554213563001No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGA5593350.137972915837809Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC5163890.12737929154544297Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAAGCCA5101640.1258437513037446No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT5051910.12461704582230426No Hit
CCTGGCCGTACGCCTAACCGCTAACATTACTGCAAGCCACCTACTCATGC4854990.11975955852278226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGA4805910.11854888679486972Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG4691910.11573681309922931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTC4659250.11493117865274147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4568510.1126928666602642Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA4490440.11076708952501291No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA4441510.1095601178940683Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAA4429100.1092539965382534Clontech SMARTer II A Oligonucleotide (100% over 21bp)
CCCGATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGC4372480.10785733326941868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)