FastQCFastQC Report
Mon 13 May 2024
EGAF00006214603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006214603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358887255
Total Bases32.2 Gbp
Sequences flagged as poor quality0
Sequence length90
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11069040.30842666731088014Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5599470.15602309421659458Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC5424080.15113604410387882Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3817710.10637630472556069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)