FastQCFastQC Report
Wed 14 Sep 2022
EGAF00006214759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006214759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304206293
Sequences flagged as poor quality0
Sequence length90
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA36635161.2042867239436101Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG31341321.0302653403688793Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC28913140.9504451638677966Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT16889580.5552015322707344Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA13339750.43850999492637055Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAG12857730.4226648263321758Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA11261030.37017741773014534Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG9415150.3094988570798567Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT8522710.28016218586247327Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGG7828600.25734510363991714Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT7146600.23492610654178678Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGC7018120.23070265676588092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGCCGCCGGTGAAATAC5809800.1909822424350702Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC5688160.1869836400787409Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGAGTGTCCCGCGGGGCC4544760.14939730388812172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG4388860.1442724920881239Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGACAG4375720.14384054836104262Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGC4064970.13362544081229774Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGA3575130.11752320981735903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC3372500.11086226937455236Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3310310.10881793296761287Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3166210.10408101583881436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGAGTGTCCCGCGGGGCCC3154590.1036990382049723Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGACA3073700.10103998736147117Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA3071440.10096569567020759Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)