FastQCFastQC Report
Wed 14 Sep 2022
EGAF00006214811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006214811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215850351
Sequences flagged as poor quality0
Sequence length90
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT26282321.2176176632670845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG15649410.7250120246503561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGC14248070.6600901936916471Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGGGCCGCCGGTGAAATAC12840960.5949010479023961Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA11631020.5388464714611467Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT10704880.49593989309750997Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG9777790.45298930276004046Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC8466080.3922198857114668Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGG7767070.3598358753653359Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGGGGGGGATGCGTGCAT7360430.3409968974291823Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTCCTACTTGGATAACTGTG6593350.3054593133369517Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGAGTGTCCCGCGGGGCCC5896770.2731878809870455Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAG5658440.2621464349622485Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA5279710.2446004824889073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGAGTGTCCCGCGGGGCC4779580.22143026304367697Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA4708250.2181256587347407Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTC4513670.20911107992592515Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTCGCCGGCCGAGGTGGGA4455700.20642542295425778Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGAGTGTCCCGCGGGGC3453910.16001410162172958Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT3449590.1598139629617744Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC3066380.14206045928551675Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT3001480.1390537465468379Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATT2951180.13672342835337803Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCAGTGGTATCAACGCAGAGTACATGGGAGCCCCTCTCCGGCCCCGGCCG2942270.13631064236722043Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCGGGCCGCCGGTGAAATACCA2784620.1290069711306608Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGCCGGGGGGCGGGCGCCG2639790.12229722989887563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGTCCCGCGGGGCCCGA2487820.11525670393744229Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGGGGCGGGCGCCGGC2234520.10352172186182824Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACCCCCGCCACGCAGTTTTA2231280.10337161786686183Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2218010.10275684008500871Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCGGCGCCTCGCCTCGGC2168370.10045709863126422Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)