FastQCFastQC Report
Mon 5 Sep 2022
EGAF00006214963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006214963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59695715
Sequences flagged as poor quality0
Sequence length90
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10239631.7153040214025412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6445141.0796654332727231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3595390.6022861104854846No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3525940.5906521096195932No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAG2470730.4138873284288495Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2277840.38157512645589386Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCAT2153480.3607428104345513Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2090350.3501675120232667Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1733820.29044295725413455Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA1257980.21073204333007822Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1161240.19452652506130463Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC941370.15769473571093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTGAGTGTCCCGCGGGGCCCG883470.1479955470840746Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC840130.14073539449188271Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGCGGCGCCTCGCCTCGG827460.13861296409633422Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAGAGTAC825080.138214275513745Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATT773580.12958719063839003Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAA759430.12721683625030708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA694100.11627300217444418Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA676540.1133314175062649Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT618480.10360542628562201Clontech Universal Primer Mix Long (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)