FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34538697
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCCCAAACCCACTCCACCTTATTACCAGACAACCTTAACCAAACC840200.24326337499066628No Hit
AGATCTCGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC578940.1676206835480794No Hit
AGATCTGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACG514460.14895176850475858No Hit
AGATCTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCA487560.1411634028927032No Hit
AGATCTGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAG402530.11654463977028431No Hit
AGATCTGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGA402030.11639987461020895No Hit
AGATCTCTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGA399070.11554286486256271No Hit
AGATCTCTTCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT378500.10958722617706164No Hit
AGATCTCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCG357750.10357947203393342No Hit
AGATCTGGCTTCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC354860.10274272940869772No Hit
AGATCTTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTA349120.10108082537103238No Hit
AGATCTCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCA346060.10019486259137107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)