FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11934697
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTACCCAGTTTCGACCTTTATTTAGGT842890.706251696209799No Hit
CCCTACTGCTAACTGGGTTCTGTTTTGC834610.6993139415269612No Hit
TCATGAGTCTGTTCATGCCTCGAGGTAG750170.6285622500512581No Hit
TTGAACGGTCCCTGTTATGATCTTCGTC622210.5213454518367747No Hit
AGGCCACCAAAGCAATAGAATTTTCAAT572360.47957648191654967No Hit
AGTGATCCAACCAATCTTAGTTAATGCT470420.3941616615821918No Hit
GGGAGATTCCTTGGAACTACCTCAATGT467780.39194962385722903No Hit
TGGGTTAAGCTCGCACAGTCGACCTCGT463670.3885058833081393No Hit
AGGGAGTTCTAGCATGATGCCGACACAT456490.3824898110107027No Hit
CGAAGTTAGAGTATACAGATTCTATCGC450360.3773535264447853No Hit
TTGTTCAGTCTGTGCGATAGCATCCATC442780.3710022969162937No Hit
CATACTTTCTGAGAAAGGTTAGTCCACA399670.33488072633934485No Hit
CCTAACCGTAGCGCTCAACAAAAGCCTC383260.32113090093531493No Hit
AAGCGTTGTTTGGGTTTTGACAATACGC368410.3086881887323993No Hit
CTAGACAAGCGATCGACCTGCTTTTGAA362660.3038703035359842No Hit
GGAGCAATCAATGCACTCGCAAACAATC361020.30249615888865883No Hit
CTACCCAAGTGAACATTGCCACGTCAAC357160.2992618916089784No Hit
GTTTGGATCGTGGACCCTATTTTCTCAA355100.2975358318690453No Hit
TGTGATGAGGCATGGTACACTATCGGTA350100.2933463664808583No Hit
CCTCCAATCGGATACTTCTTCAACCTTG349250.2926341573648665No Hit
GTAACACAGTCTAACCTTAATTCGTCCT340360.2851852879046699No Hit
ATCTCTACAGCTAACTAAACTCCCGTGA339110.28413792155762313No Hit
TTTGACTTCTGTCGCTATGTAAAGGTAT322290.2700445599917618No Hit
AGTAACCAGACGCTCCGTAGTTTTCCAG321670.26952506628362666No Hit
CCGATGGCAACAAGTACACTACGTGCGT317730.26622376755773525No Hit
AGAGCAGTCCTCTCTTACTTGTGGTTCC313550.2627213744932108No Hit
AAGCATCTCGCAGTTATCGCTAGGCGAA311880.26132209305355636No Hit
CCACAAACATCTAGACCCAATTAACTGC307620.257752668542821No Hit
ACGTAACTCTCATTACCCCTTTCCCTAA302040.25307722516960424No Hit
TTTCATGGTGTTCCAATCTAGCCTGCCC300960.2521723006457558No Hit
CTGAGGCGTGGCATCCTCTAGAGTCGCC298710.25028704122107165No Hit
CTCTCGACATCAACCAGCTGTTATTCAT289900.24290520320708603No Hit
GGTGGCTCAATGAACACTTCGGACCGGT287550.2409361544746381No Hit
TTCACGCCATACATCGCGCCCCTGTCAT282820.23697292021741312No Hit
TGAGCGCCACTCCCTACACATTCCAATT270790.22689306649343507No Hit
TAAGCCAAGATGCCGACTTAAACTTTTA258510.21660373950004763No Hit
GTGCGTGAGATCCCATAGAGAAGACGAG241790.2025941672419501No Hit
AGAGCCCAGCACGGATGTCCACTCACAC240410.20143787479481048No Hit
CAACAGTGTGAACCGAACTAGGACTTTG236290.1979857553149443No Hit
TCCCATGTCGTGGCGTCTTATCATCGTT235730.19751653519146736No Hit
GTGCGTGGTTAAGAACTCGTAACACCCA225920.18929680409984434No Hit
TTTCACACAAGTAGTATCGCATTTTAGT223890.1875958811522404No Hit
CCAAGCGCATGATGCTTCCCACCCTGAT222850.18672447235149747No Hit
TCTACCGGTGTTCATGACGATCATATGT219600.1840013198491759No Hit
AACACACGTGAGTTGGTTCAGTTGCGTT208680.17485152744137533No Hit
GCCGTGAAGTATGGCGTACAATTCTTAA198240.16610392371084076No Hit
TTTGTTGCACTGGAAGAACACTTACTTG197750.16569335610279842No Hit
GAGTTTGCACCGAATTTACACACAGTAA194850.16326346617764992No Hit
TCACATTCACGCAGTCGGCGTAAGGTTT193490.16212393159206304No Hit
GTTCATTTCTTACACTTAGGCCTGCCAC193100.16179715329178446No Hit
CTGCTCACAACTCCCTAGAATACCAAAA192750.16150389071461135No Hit
AGATAGACAAACTGCTCAATTATCAATT189820.15904886399713375No Hit
GCATCTCGTTCGGCGTACAAATCGCCCA189800.15903210613558097No Hit
AGACCATAGGACTTCTCAGCTGTGACGA187670.1572473938802133No Hit
GGGAGTAGTTGTTGACCGTTACTTATTT178490.14955553542750183No Hit
TGGGAGAGTGCCTTCTTATGTGCCATGC176230.1476618970720413No Hit
CAACCTCGTATTGGCTCCATCCTCGTTA176050.14751107631806656No Hit
AGCCAGCTCGCACTCTAATTAATGCAAA174130.1459023216090027No Hit
TCTGCCAAGGCATGCAAAGTCTCAATGA174050.14583529016279173No Hit
GGGTGTCTCACGGGAAATAATCATGGGG173770.14560068010105326No Hit
GTGCACGGTCGGATTTTGAGGAAGGCTC171180.14343053702997235No Hit
TTCCTCTCAGAGAGGGCTCCCACTTATT162680.13630844587005436No Hit
GCAGCTGTCGGAGATGACAACGTTACCT162240.13593977291589388No Hit
CCTCTCCGTGGATGTTTTTTTTTTTTTT161760.1355375842386279No Hit
GAGAGGTTCTCTTAACCCGATTATAGCA157690.13212735941264367No Hit
CTGGCAGTCTAGTGACGCGCGTAGATCC156090.1307867304884238No Hit
GCCAACGTCCTTGAAGTTTTCCTCCAGC154290.12927852294867645No Hit
CGACAGCGTTGTGGCCTATTTGGTCTCA152990.12818926194774782No Hit
CAGCAATCATTCACCCAGCCCTTTCAGT152210.12753570534719064No Hit
TAAGCGTGTTCCAAACAATAGTCGATAC150820.12637103396927463No Hit
CGGAATTGTGAGTAATAAACTTGGGGGA148990.12483768963719817No Hit
GACAGCCAGCCACTCGCTATCCTATTAA148390.12433495379061571No Hit
GTAGGTTCATACTTTCAACCTTTAATGA147590.12366463932850578No Hit
GGTTCTCTCGCGTAGCCTTTGCACCTAG144690.12123474940335728No Hit
AGGAAATTCGTCGCTTTCGGCCTCCCGT141840.11884675413209066No Hit
TTTGGTTAGGTGCAGTTTTTGGCAGGGT140190.11746423055398893No Hit
CCTGTTGCACAATTCGATGTGAATGCTT136820.11464053088235085No Hit
GGTCTGGCAAACTCGTTGTTCCCACATA136730.11456512050536348No Hit
AACAAGATCATTCTTGCCGTCCCGATAT136090.11402886893567554No Hit
TCACTATCAGTATTCGTACACCTATGCA133950.11223577774953147No Hit
TTGTTCAGTCTGTGCGATTCCCTAATTC133620.11195927303391112No Hit
AAGAACAGTTGTGCCGCCTAATTGAGAG133560.1119089994492529No Hit
TCTTGCGGTGTAAATGTTTAGCTCAAAG132610.11111300102549734No Hit
GATTTCTAGAAGAACGACCGCTACTGCG130710.10952100417798624No Hit
GACCAATGTGTAGTGGCACTCGGCATTG130440.10929477304702415No Hit
GGCTTTCAGCAACCAGGCCGCAAGGCAG129450.1084652589001631No Hit
GTAGCTACAAATTGCCTCTTTTTAGACG129180.10823902776920101No Hit
CTACTATCAAGAGGCTACATTTAACATA127280.10664703092168992No Hit
TCGGGACTCGGCTGACACTCCTTCACTG127100.1064962101677152No Hit
CTACATTAGCCGAATGCTAGTCCATGGT124550.10435958281973978No Hit
GCCATGGTCTAGCAACTATACGTGTCAC123890.1038065733884991No Hit
TACTTACTCTAGCCAAGCACATGGGAAT123240.10326194288803477No Hit
ACAGCCGCAGGTGGATAGACCTCTGAAA123180.10321166930337654No Hit
CGGAACCAGGTCATCTTCTTGTTAAAGA121970.10219781867943525No Hit
CGCCATTAGGGACACTTATTACTCCGAT121540.10183752465605117No Hit
AGGACTTAGCCTGACCGTATTCTAATCA121250.10159453566353632No Hit
TAGCACAAGCGACCCTTCACTATGTGCA120870.1012761362940341No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)