FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8762
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG370942.330518146541884No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACAGGG156517.86121889979457No Hit
GGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT96411.002054325496461No Hit
ATGACCTGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG1872.134215932435517No Hit
ATGACCGGCCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG1691.9287833827893175No Hit
ATGACCGGCCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGG1211.3809632503994522No Hit
ATGACCTGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACAGGG1011.1527048619036748No Hit
ATGACCGGCCCAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG881.0043369093814198Clontech SMART CDS Primer II A (100% over 26bp)
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCAACGAGACAGGG860.9815110705318421No Hit
GGTTTCCCAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGG740.8445560374343757No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACAGAG530.6048847295138097No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACCGGG530.6048847295138097No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG360.41086509929239895No Hit
ATGACCTTCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG300.3423875827436658No Hit
ATGACCGTCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG270.30814882446929925No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGATACCGAG220.25108422734535496No Hit
ATGACCTTCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACAGGG210.23967130792056607No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTACGAGCCCACGAGACAGGG200.22825838849577723No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCACACGAGACCGAG180.20543254964619947No Hit
ATGACCGTCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACAGGG180.20543254964619947No Hit
GGTTTCCCAGTCCATATACACATCTCCGAGCCCACGAGACCGAGGCTGATC160.18260671079662177No Hit
ATGACCTGCCCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG150.1711937913718329No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCAGAGCCCACGAGACAGGG120.13695503309746632No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCAACGAGACCGAG120.13695503309746632No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGATAGGG120.13695503309746632No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGAAAGGG120.13695503309746632No Hit
GGTTTCCCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC120.13695503309746632Clontech SMARTer II A Oligonucleotide (95% over 23bp)
ATGACCGGCCAAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG110.12554211367267748No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCATCAATCATCACAGGGT110.12554211367267748No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGATACAGGG110.12554211367267748No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCAGAGCCCACGAGACCGAG110.12554211367267748No Hit
GGTTTCCCAGTCACTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC110.12554211367267748No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCATGAGACAGGG110.12554211367267748No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCCCGAGCCCACGAGACCGAGG110.12554211367267748No Hit
ATGACCGGCCCAGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGT100.11412919424788862Clontech SMART CDS Primer II A (100% over 26bp)
GGTTTCCAGTCCACTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC100.11412919424788862No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGATCCCACGAGACCGAG100.11412919424788862No Hit
GGTTTCCCAGTCCACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG100.11412919424788862Clontech Universal Primer Mix Long (96% over 25bp)
ATGACCGGCCCAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT90.10271627482309974Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATGACCGGCCCAGTACTCTGCGTTGATACCACTGCTTGGCCATGTACTCTG90.10271627482309974Clontech Universal Primer Mix Long (96% over 27bp)
GTGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG90.10271627482309974No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)