FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8762
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGAATAGA193622.095412006391236No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110612.62268888381648No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACTGACGCTGCCGACGAATAGA2352.6820360648253825No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGAATATA961.0956402647797305No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCAGACGCTGCCGACGAATAGA931.0614015065053641No Hit
GACTGGGAAACCCTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAG760.8673818762839535No Hit
CTGGGCCGGTCAACTGTCTCTTATACACATCTGACGCTGCCGACGAATAGA480.5478201323898653No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCAGCCGACGAATAGA480.5478201323898653No Hit
CTGGGCCGGTCATCTGTCTCTTATAAACATCTGACGCTGCCGACGAATAGA450.5135813741154988No Hit
GACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCGGCT370.42227801871718784No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATATGACGCTGCCGACGAATAGA330.37662634101803244No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTTACGCTGCCGACGAATAGA320.36521342159324355No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACAGACGCTGCCGACGAATAGA310.3538005021684547No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGAAAAGA270.30814882446929925No Hit
GTCCAACCCTGTGATGATACCACTGCTTCCATGTACTCTGCGTTGATACCA260.2967359050445104No Hit
GACTGGCACGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA250.2853229856197215Clontech Universal Primer Mix Long (96% over 26bp)
CTGGGCCGGTCATCTGTCTCTTATACACAACTGACGCTGCCGACGAATATA240.27391006619493263No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTTCCGACGAATAGA190.21684546907098837No Hit
GTCCAACCCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA190.21684546907098837Clontech Universal Primer Mix Long (95% over 23bp)
CTGGGCCGGTCATCTGTCTCTTATACACAACTGACGCAGCCGACGAATAGA170.19401963022141064No Hit
GTCCAACCCTGTGATGATACCACTGCTTGTACATGGAAGCAGTGGTATCAA170.19401963022141064No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCAGACGCAGCCGACGAATAGA160.18260671079662177No Hit
CTGGGCCGGTCAACTGTCTCTTATACACAACTGACGCTGCCGACGAATAGA160.18260671079662177No Hit
CTGGGCCGGTCATCTGTATCTTATACACATCTGACGCTGCCGACGAATAGA150.1711937913718329No Hit
CTGGGCCGGTCATCAGTCTCTTATACACATCTGACGCTGCCGACGAATAGA140.15978087194704405No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCAGACGCTGCCGACGAATATA130.1483679525222552No Hit
GTCCAACCCTGTGATGATACCACTGCTTGTACTCTGCGTTGATACCACTGC130.1483679525222552Clontech SMART CDS Primer II A (95% over 24bp)
CTTGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGAATAGA130.1483679525222552No Hit
GTCCAACCCTGTGATGATTGATGGAGATGTGTATAAGAGACTGCTGGGCCG120.13695503309746632No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGAAGCTGCCGACGAATAGA120.13695503309746632No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCCACGAATAGA110.12554211367267748No Hit
CTCCAACCCTCAAAAGTATCATATAAAAATATTAAAATACACACCAATAGA110.12554211367267748No Hit
CTCCAACCCTCAAAAGTCTCATAAAAAAATATTAAAAAAAACAACAATAGA100.11412919424788862No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACAGACGCAGCCGACGAATAGA100.11412919424788862No Hit
CTGGGCCGGTCATCTGTCTCTTAAACACATCTGACGCTGCCGACGAATAGA100.11412919424788862No Hit
GACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCT100.11412919424788862No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAAATGACGCTGCCGACGAATAGA100.11412919424788862No Hit
GACTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGAATA100.11412919424788862No Hit
CTGGGCCGGTCATCTGTCTCTTATAAACAACTGACGCTGCCGACGAATAGA100.11412919424788862No Hit
CTCCAACCCTCAAAAGTCTCATAAAAAAAAATTAAAAAAAACAACAATAGA90.10271627482309974No Hit
GTCCAACCCTGCGTTGATACCACTGCTTGGCCATGTACTCTGCGTTGATAC90.10271627482309974Clontech Universal Primer Mix Long (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)