FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14509
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG1186281.75615135433179No Hit
GGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACGCTACGCTAT8716.003170445930112No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACGCTA2581.7782066303673583No Hit
GGTTTCCCAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTAC660.45489006823351025No Hit
GGTTTCCCAGTCCATATACTGACGTCTCCAACATGAGCTGCTTGGCGAGGC480.33082914053346196No Hit
GGTTTCCAAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG430.296367771727893No Hit
GGTTTCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGGC370.25501412916121025No Hit
GGTTTCCCAGTCAACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG340.2343373078778689No Hit
GGATTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG330.22744503411675512No Hit
TGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG310.21366048659452755No Hit
GGTTTCCCAGTCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGGC270.18609139155007237No Hit
GGTTTCCCAGTCCACTTACTGACGTCTCCAACATGAGCTGCTTGGCGAGGC230.1585222965056172No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG220.1516300227445034No Hit
GGTTTCCCAGTCCACATACTGACGTCTCCAACATGAGCTGCTTGGCGAGGC210.14473774898338962No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCAAACATGAGCTGCTTGGCGAGG210.14473774898338962No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATTAGCTGCTTGGCGAGG210.14473774898338962No Hit
GGTTTCCCAGTCCACTATACTGACGTCTACAACATGAGCTGCTTGGCGAGG180.12406092770004824No Hit
GGTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGGC180.12406092770004824No Hit
GGTATCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG180.12406092770004824No Hit
GGTTTCCCAGTCCACTATACTTACGTCTCCAACATGAGCTGCTTGGCGAGG170.11716865393893446No Hit
GGTTTCCCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC170.11716865393893446Clontech SMARTer II A Oligonucleotide (95% over 23bp)
ATGACCTGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACGCTA150.10338410641670688No Hit
GGTTTCACAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG150.10338410641670688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)