FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14509
Sequences flagged as poor quality0
Sequence length51
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCT1192182.1627955062375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9596.6096905369081265No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGAATAGA1270.8753187676614516No Hit
GACTGGGAAACCCTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAG710.4893514370390792No Hit
GACTGGCACGCTCCATTACCGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCT400.27569095044455166No Hit
GACTGGCACGCTCCGTGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCT350.2412295816389827No Hit
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT310.21366048659452755Clontech SMART CDS Primer II A (96% over 26bp)
GACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCGGCT280.19298366531118616No Hit
GACTGGCACGCTCCATGACCGGCCCAACAGTCTCTGCCTCGCCAAGCAGCT270.18609139155007237No Hit
GACTGGCACGCTCCATGACCGGCCAAGCAGTCTCTGCCTCGCCAAGCAGCT240.16541457026673098No Hit
GACTGGCACGCTCAATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCT200.13784547522227583No Hit
TACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCT200.13784547522227583No Hit
GACTGGCACGCTCCATGACCGGCCCAGCAGTCTATGCCTCGCCAAGCAGCT180.12406092770004824No Hit
CACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCT170.11716865393893446No Hit
GACTGGCACGCTACATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCT170.11716865393893446No Hit
GACTGGCACGCTCCATGACCGGCCCAGTAGTCTCTGCCTCGCCAAGCAGCT160.11027638017782067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)