FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192
Sequences flagged as poor quality0
Sequence length51
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTGGGAAACCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCA6734.89583333333333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6131.770833333333332No Hit
GACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCGGCT199.895833333333332No Hit
GACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCT94.6875No Hit
GACTGGGAAACCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGAA21.0416666666666665No Hit
GACTGGCACGCTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT21.0416666666666665Clontech Universal Primer Mix Long (96% over 26bp)
GGACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCGGC10.5208333333333333No Hit
GACTGGGAACCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAG10.5208333333333333No Hit
TCTCTCACTCTGTTCTTAGCTGTGCTCGTTGATGGTATGCAAAGGTCACCG10.5208333333333333No Hit
GACTGGCACGCTCCATGAACGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCT10.5208333333333333No Hit
GCCTCAGCCTCCTGAGTAGCTGGGATTACAAGTGTGTGCCACCACACCTGG10.5208333333333333No Hit
GACTGGCACGCTCCATGACCGGCCCAGCAGTCTATGCCTCGCCAAGCAGCT10.5208333333333333No Hit
CTCTTGAAGAAGCCGACAAGATGTTTCTGAGAACAAGAGAACCAGCCCTGG10.5208333333333333No Hit
TACTGGGAAACCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCA10.5208333333333333No Hit
GTAGGATAAGTGGGAGCCGAAAGGCGAAAGTGAAATACCACTACTTTTAAC10.5208333333333333No Hit
CTCCAACCCTCAAAAGTCTCTTATACACATCTGAATCTTCAGACGATATGC10.5208333333333333No Hit
CCATGAAGAAGCTTCATCTCAAGCTCCGCCTGCCCCCTCAGCAGAAGCCCC10.5208333333333333No Hit
GTCCAACCCTGTGATGATTGATGCTTGGCCGGTCATCTGTCTCTTATACAC10.5208333333333333No Hit
GACTGGGAAACCCTGTCTCTTATACACATCTGAGCTGCCGACGATATGCAG10.5208333333333333No Hit
ACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCTC10.5208333333333333No Hit
GACTGGCAAACCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCA10.5208333333333333No Hit
GACTGGTAAACCATGTCTCTTATACACATCTGACGCTGCCGACGATATGCA10.5208333333333333No Hit
CCCTTGGCTGGAGCTCACTGGCTGCCTTCCTGCCACCTGTAAGCCTCGGCA10.5208333333333333No Hit
GACTGGCACGCTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGAACT10.5208333333333333Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAACCCTGTGATGATTGCTGGGCCGGTCATCTGTCTCTTATACACATC10.5208333333333333No Hit
GACTGGAAGCGAGGGGTAACGGGTGATGGGCAAAAAGCTTCAGCAGACAGA10.5208333333333333No Hit
CCCAAGTGGCTGGAACTATAGGCGCGTGCCACCATGCCCTGCTAATTTTTT10.5208333333333333No Hit
GTCAAACCCTGTGATGATTGATGCTGGGCCGGTCATCTGTCTCTTATACAC10.5208333333333333No Hit
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTCC10.5208333333333333No Hit
GACTGGGAAAACCATTATCTTATAAACAACATAAGCAGCCCACCATAAGAC10.5208333333333333No Hit
GACGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCGGCTC10.5208333333333333No Hit
CCACAAGAACAAGCTCTTGGTCAGCCACACTGCAAAGTGACACAGTGGTGA10.5208333333333333No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGATATGC10.5208333333333333No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACTGACGCTGCCGACGATATGC10.5208333333333333No Hit
CCTGGGAAACCCTGTCTCTTATACACAAAAGACGCTGCCCACGATATGCAG10.5208333333333333No Hit
CAGTGCCTGTCAACATTAACTTAAAAACAAAATAAGCATCCCAACAAATGA10.5208333333333333No Hit
GACTTGGAAACCCTGTCTCTTATACACATCTGGACGCTGCCGACGATATGC10.5208333333333333No Hit
CCTGAGATGACTGAATATGAACCAAAGCTGCACTGTGCTGTACTTTTCAGC10.5208333333333333No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)