FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758
Sequences flagged as poor quality0
Sequence length51
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGATATGC52218.92675852066715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1655.982596084118927No Hit
GACTGGGAAACCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCA853.0819434372733863No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACTGACGCTGCCGACGATATGC722.610587382160986No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCAGACGCTGCCGACGATATGC471.704133430021755No Hit
CTGGGCCGGTCATCTGTCTCTTATAAACATCTGACGCTGCCGACGATATGC351.2690355329949239No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCAGCCGACGATATGC260.9427121102248005No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACAGACGCTGCCGACGATATGC260.9427121102248005No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGATATTC230.8339376359680928No Hit
GACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCGGCT150.5438723712835388No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTTACGCTGCCGACGATATGC140.5076142131979695No Hit
CTGGGCCGGTCATCTGTCTCTTATAAACAACTGACGCTGCCGACGATATGC120.43509789702683105No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTTCCGACGATATGC90.3263234227701233No Hit
CTGGGCCGGTCATCTGTCTCTTAAACACATCTGACGCTGCCGACGATATGC90.3263234227701233No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATATGACGCTGCCGACGATATGC90.3263234227701233No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCAGACGCAGCCGACGATATGC90.3263234227701233No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCAGACGCTGCCGACGATATTC90.3263234227701233No Hit
GACTGGCACGCTCCATGACCGGCCCAGCAGTCTCTGCCTCGCCAAGCAGCT80.290065264684554No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGAAGCTGCCGACGATATGC80.290065264684554No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACAGACGCAGCCGACGATATGC80.290065264684554No Hit
CTGGGCCGGTCAACTGTCTCTTATACACATCTGACGCTGCCGACGATATGC70.25380710659898476No Hit
CTGGGCCGGTCAACTGTCTCTTATACACATCAGACGCTGCCGACGATATGC70.25380710659898476No Hit
CTGGGCCGGTCATCTGTCTCTTATAAACATCAGACGCTGCCGACGATATGC60.21754894851341552No Hit
CTGGGCCGGTCCTCTGTCTCTTATACACATCTGACGCTGCCGACGATATGC60.21754894851341552No Hit
GTCCAACCCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA60.21754894851341552Clontech Universal Primer Mix Long (95% over 23bp)
GTCCAACCTGGTGCGAATAAACTGTAGTACCAGCATTGTGGCAATTAGGTC50.18129079042784627No Hit
CTTGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGATATGC50.18129079042784627No Hit
CTCCAACCCTCAAACGTATCCTATAAAAATATTAAAATACACACCATATGC50.18129079042784627No Hit
CTGGGCCGGTCATCTGTCTCTTAAACACAACTGACGCTGCCGACGATATGC50.18129079042784627No Hit
GACTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGATAT50.18129079042784627No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACTGACGCAGCCGACGATATGC50.18129079042784627No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCAGCCGACGATATTC50.18129079042784627No Hit
CTGGGCCGGTCATCAGTCTCTTATACACATCTGACGCTGCCGACGATATGC50.18129079042784627No Hit
CTGGGCCGGTCATCTGTCTATTATACACATCTGACGCTGCCGACGATATGC40.145032632342277No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACTGACGCTGCCGACGATATTC40.145032632342277No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATATTACGCTGCCGACGATATGC40.145032632342277No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCCACGATATGC40.145032632342277No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCATACGCTGCCGACGATATGC40.145032632342277No Hit
GTCCAACCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAGTGT40.145032632342277No Hit
CTCCAACCCTCAAAAGTCTCATAAAAAAAAATTAAAAAACCCAACATATGC30.10877447425670776No Hit
GACTGGGAAACCCTGTCTCTTATACAATCTGACGCTGCCGACGATATGCAG30.10877447425670776No Hit
CTCCAACCCTCAAACGTCTCATAAAAAAATATTAAAAAACCCACCATATGC30.10877447425670776No Hit
CTGGGCCGGTCATCTGTCTCTTATAAACAACAGACGCTGCCGACGATATGC30.10877447425670776No Hit
CTGGGCCGGTCATCTGTCTCTTAAACACATCTGACGCAGCCGACGATATGC30.10877447425670776No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAAATGACGCTGCCGACGATATGC30.10877447425670776No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTTACGCTGCCGACGATATTC30.10877447425670776No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACTTACGCTGCCGACGATATGC30.10877447425670776No Hit
GACCAACCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAGTGT30.10877447425670776No Hit
CTCCAACCCTCAAAAGTCTCATAAAAAAAAATTAAAAAACACAACATATGC30.10877447425670776No Hit
CTGGGCCGGTCAACTGTCTCTTATACACAACTGACGCTGCCGACGATATTC30.10877447425670776No Hit
CTCCAACCCTCAAAAGTCTCATAAAAAAAAATTAAAAAAAACAACAAATGC30.10877447425670776No Hit
CTGGGCCGGTCATCTGTCTCTTAAACACATCAGACGCTGCCGACGATATGC30.10877447425670776No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACAGACGCTGCCGACGATATTC30.10877447425670776No Hit
CTGGGCCGGTCATCTGTCTCTTATAAACATCTTACGCTGCCGACGATATGC30.10877447425670776No Hit
CTTGGCCGGTCATCTGTCTCTTATACACAACTGACGCTGCCGACGATATGC30.10877447425670776No Hit
CTGGGCCGGTAATCTGTCTCTTATACACATCTGACGCTGCCGACGATATGC30.10877447425670776No Hit
CTGGGCCGGTCATCTGTCTCTTATACACAACATACGCTGCCGACGATATGC30.10877447425670776No Hit
CTCCAACCCTCATATGAATCATATACACATCTCACACATCAGAATAAAATA30.10877447425670776No Hit
CTGGGCCGGTCATCTGTCTCTTATAAACATCTGACGCTTCCGACGATATGC30.10877447425670776No Hit
CTCCAACCCTCAAACGTCTCATAAAAAAATATTAAAAAACACAACATATGC30.10877447425670776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)