FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4149
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG292570.49891540130152No Hit
GGTTTCCCAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGG49912.02699445649554No Hit
GGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACTAAGGCGAAT1533.68763557483731No Hit
ATGACCGGCCCAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT1253.0127741624487827Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG250.6025548324897566No Hit
ATGACCTGCCCAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT130.31332851289467345Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTTTCCAAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG110.2651241262954929No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACTAAG110.2651241262954929No Hit
GGATTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG80.1928175463967221No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATTAGCTGCTTGGCGAGG80.1928175463967221No Hit
GGTTTCCCAGTCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGGC70.16871535309713184No Hit
GGTTTCACAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG60.14461315979754158No Hit
GGTTTCCAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC60.14461315979754158No Hit
TGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG60.14461315979754158No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGTTGCTTGGCGAGG60.14461315979754158No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCAAACATGAGCTGCTTGGCGAGG60.14461315979754158No Hit
GGTTTCCCAGTCAACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG60.14461315979754158No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGGGCTGCTTGGCGAGG50.1205109664979513No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTTGCGAGG50.1205109664979513No Hit
GGTTTCCCAGTCCTGTCTCTTATACACATCTCAGAGCCCACGAGACTAAGG50.1205109664979513No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGATGCTTGGCGAGG50.1205109664979513No Hit
GGTTTCCCAGTCCTGTCTCTTATACACATCTCGAGCCCACGAGACTAAGGC50.1205109664979513No Hit
GGTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGGC50.1205109664979513No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGATG50.1205109664979513No Hit
GGTTTCCCAGTCCACATACTGACGTCTCCAACATGAGCTGCTTGGCGAGGC50.1205109664979513No Hit
GGTTTTCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG50.1205109664979513No Hit
GGTATCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG50.1205109664979513No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)