FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893728
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCT21820.2441458698843496No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTG20750.23217354720899425No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCT20580.23027140248487238No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATACCAA19990.22366984138350818No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTG19600.21930609760464037No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGT17500.19580901571842885No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGT17180.19222850800243474No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTC15720.17589244154821154No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTG15220.17029789824197072Clontech SMART CDS Primer II A (96% over 26bp)
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCT14730.16481524580185472No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGT14720.16470335493572988No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGT14710.16459146406960506No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTC14070.15743044863761682No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCG13930.15586397651186937No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTCT13620.15239535966200007No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG12870.14400354470263885Clontech Universal Primer Mix Long (96% over 25bp)
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGG12790.1431084177736403No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATAC12160.13605929320777685No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGG11270.1261010061226682No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG9660.10808657667657273Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)