FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439799
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCT42360.9631672650460779No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTG40970.931561918058022No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCT40520.9213299711913852No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTG39710.902912466831439No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGT36600.8321983451531267No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGT36510.8301519557797994No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTC33880.7703519107592331No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATACCAA32010.7278324871134313No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCT31800.7230575785756675No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGT31300.7116887487238489No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGT30760.6994104124838847No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTC30210.6869046996468842No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTCT29660.6743989868098836No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATAC27320.6211928631033722No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGG23870.5427479371258234No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGG22200.504776045420749No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCG21750.49454409855411224No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATA16010.36402993185523386No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC13060.29695383572950373No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCT10650.24215607584373772No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCC10140.23055986939488265No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCC9790.22260168849860962No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCT9580.21782677996084573No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCTG9390.21350662461715464No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCGGGTTTGACCGCTCGCC9390.21350662461715464No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTCTGTCTCTTATAC9340.21236974163197278No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTG9180.2087317160793908No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCCTGTCTCTTATAC8930.2030473011534815No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC8770.1994092756008995No Hit
AGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG8570.19486174366017203No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT8330.18940470533129908No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAA8320.18917732873426268No Hit
CATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCAT8260.18781306915204446No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACA8040.18281078401724424No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT8040.18281078401724424No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT7970.18121914783798965No Hit
ACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACG7970.18121914783798965No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA7950.1807643946439169No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC7730.17576210950911667No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA7540.17144195416542557No Hit
GATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGA7330.16666704562766171No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA7240.16462065625433436No Hit
GTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAAC7210.16393852646322524No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG7200.16371114986618887No Hit
CCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCC7120.1618921370898979No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCT7050.16030050091064327No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC7040.1600731243136069No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT7040.1600731243136069No Hit
GCAATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTACACGACCGGGG6630.15075068383511558No Hit
ACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATC6560.14915904765586097No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA6520.14824954126771547No Hit
CCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACA6420.14597577529735173No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCTT6400.145521022103279No Hit
CCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC6350.14438413911809714No Hit
CCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCT6260.14233774974476976No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCTGTCTCTTATACACATC6250.1421103731477334No Hit
CTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCC6160.14006398377440604No Hit
GTAGATGTGGTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCG6150.13983660717736968No Hit
CCTTTAATAGCGGCTGCACCATTGGGATGTCCTGATCCAACATCGAGGTCG6080.13824497099811506No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTCT6010.13665333481886044No Hit
GATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGT5940.13506169863960582No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTTA5880.1336974390573876No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA5780.13142367308702385No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTCTGTCTCTTATACACATC5690.1293772837136965No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC5690.1293772837136965No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGA5630.12801302413147825No Hit
GGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAA5550.12619401135518726No Hit
ATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCT5510.12528450496704174No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCT5440.12369286878778714No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCT5400.12278336239964166No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTC5320.12096434962335066No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC5250.11937271344409604No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCTT5230.11891796025002331No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATCTGTCTCTTATACACA5210.11846320705595056No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATT5200.11823583045891417No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTCT5190.11800845386187782No Hit
GGTAGGTGGTAGTTTGTGTTTAATATTTTTAGTTGGGTGATGAGGAATAGT5150.11709894747373231No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT5130.11664419427965957No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG5050.11482518150336858No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCAT5040.11459780490633222No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTC5040.11459780490633222No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTT5020.11414305171225947No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAA4990.11346092192115036No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCCTGTCTCTTA4940.11232403893596848No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTC4930.1120966623389321No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTTTGACTATTTCTGTATGTCTCC4900.11141453254782299No Hit
CAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGTCTC4780.1086860133833865No Hit
GCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT4740.10777650699524101No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCTTA4740.10777650699524101No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA4710.10709437720413188No Hit
GGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACG4660.10595749421895002No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTCTT4570.10391110484562266No Hit
CCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGA4500.10231946866636804No Hit
CCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGACGATGGGCATGAA4490.10209209206933167No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGCTGTCTC4420.10050045589007706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)