FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439799
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCT46081.047751359143609No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTG43600.9913619630785883No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTG42370.9633946416431142No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCT40870.9292881520876582No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGT39350.8947269093381295No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGT38740.8808569369189106No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTC34060.7744446895058879No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGT32990.750115393622996No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATACCAA32990.750115393622996No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTC32530.7396560701593228No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCT32250.7332895254423043No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTCT31850.7241944615608494No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGT29640.6739442336158108No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGG25640.5829935948012616No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATAC25500.5798103224427522No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGG25450.5786734394575703No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCG23660.5379730285880595No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATA17080.3883592277381258No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGC12950.2944526931621036No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCC11460.26057358020368393No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCT11300.25693555465110196No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC11210.2548891652777746No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTG10120.23010511620080992No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCGGGTTTGACCGCTCGCC10030.22805872682748257No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCT9750.2216921821104641No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTCTGTCTCTTATAC9600.2182815331549185No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCCTGTCTCTTATAC9360.21282449482604554No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCTG9320.21191498843790005No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCC8920.20281992455644512No Hit
AGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG8550.1944069904660993No Hit
GTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAAC8530.19395223727202654No Hit
ACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACG8530.19395223727202654No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT8380.19054158831648094No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAA8310.18894995213722632No Hit
CATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCAT8300.18872257554018995No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT8220.18690356276389897No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA8070.18349291380835336No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC7980.181446524435026No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA7970.18121914783798965No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACA7800.17735374568837128No Hit
CCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCC7240.16462065625433436No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC7110.16166476049286152No Hit
GCAATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTACACGACCGGGG7000.1591636179254614No Hit
CCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACA6990.15893624132842504No Hit
GATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGA6970.15848148813435228No Hit
ACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATC6870.15620772216398854No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT6850.1557529689699158No Hit
CCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC6760.15370657959658843No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA6710.15256969661140657No Hit
CTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCC6660.1514328136262247No Hit
CCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCT6640.15097806043215195No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT6610.15029593064104285No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCTT6490.14756741147660635No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGCT6390.14529364550624263No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA6320.143702009326988No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG6310.14347463272995165No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTCTGTCTCTTATACACATC6280.14279250293884252No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6210.1412008667595879No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA6180.14051873696847877No Hit
CCTTTAATAGCGGCTGCACCATTGGGATGTCCTGATCCAACATCGAGGTCG6140.13960923058033328No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCT6110.13892710078922416No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAA6070.1380175944010787No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCTGTCTCTTATACACATC6040.13733546460996954No Hit
GTAGATGTGGTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCG5960.13551645183367855No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCTTA5910.1343795688484967No Hit
ATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCT5890.13392481565442396No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGA5830.1325605560722057No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTCT5810.13210580287813298No Hit
GATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGT5690.1293772837136965No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTATACACA5630.12801302413147825No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCT5600.12733089434036912No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTCT5580.12687614114629636No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCTT5580.12687614114629636No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTC5460.12414762198185988No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTC5440.12369286878778714No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTA5400.12278336239964166No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTCTT5370.12210123260853252No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTC5340.12141910281742341No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAAAAA5270.11982746663816879No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC5250.11937271344409604No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC5220.11869058365298693No Hit
GGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAA5200.11823583045891417No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTTA5170.11755370066780506No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATT5150.11709894747373231No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC5090.11573468789151409No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTT4950.11255141553300485No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGCTGTCTC4850.11027764956264112No Hit
CCCAGTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAA4770.10845863678635012No Hit
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCAC4740.10777650699524101No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA4740.10777650699524101No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTTTGACTATTTCTGTATGTCTCC4720.10732175380116825No Hit
CAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGTCTC4700.10686700060709553No Hit
ACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAAC4630.10527536442784091No Hit
CCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCATTTGGATACATAG4530.10300159845747715No Hit
CCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGA4520.10277422186044079No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT4520.10277422186044079No Hit
GATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGT4500.10231946866636804No Hit
GGTAGGTGGTAGTTTGTGTTTAATATTTTTAGTTGGGTGATGAGGAATAGT4460.10140996227822256No Hit
ACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCC4440.10095520908414982No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCAT4430.10072783248711342No Hit
GATGAATCCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCC4410.10027307929304069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)