FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737241
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTTAAGTACTCTGCGTTGATACCACTGCTTAAGTACTCTGCGTTGATA27610.37450440222396747Clontech SMART CDS Primer II A (100% over 26bp)
CTTAAGCAGTGGTATCAACGCAGAGTACTTAAGCAGTGGTATCAACGCAGA20730.2811834935930042Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACTTAAGCAGTGGTATCAACGCAGAGTACTTAAGC17090.23181022216615735Clontech SMART CDS Primer II A (100% over 26bp)
GCGTTGATACCACTGCTTAAGTACTCTGCGTTGATACCACTGCTTAAGTAC16510.22394305254319824Clontech SMART CDS Primer II A (100% over 26bp)
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATACCAA15920.21594024206467083No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTG13810.18732002153976787No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTG13170.17863900678339917No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCT13090.1775538799388531No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGT12860.1744341402607831No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGT11980.16249774497077618No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCT11770.1596492870038427No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTC11620.1576146741703188No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTCT11140.15110391310304228No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCT11120.15083263139190575No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTC10280.13943879952417187No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGG10120.1372685458350797No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCG9470.12845189022314277No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGT9280.12587471396734584No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGT9010.12221241086700278No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGG8860.12017779803347889No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATAC8590.11651549493313584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)