FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12511537
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACTGAGTTCAACCTAGGCTAGTAAT1862611.488713976548205No Hit
CAGCAATCAAATACAGGGTGATTCATCT1507611.204975855484422No Hit
ACTTTCAGTATGATCCCGGTCAACACCG1398961.1181360051926472No Hit
CTCAAGACACTTTATCGCAAAGCGGTCA1220280.9753238151315862No Hit
CAACGATAGACGAGCTAATACCTTCATG1000980.8000455899223253No Hit
CGAATTGAGAGTCTTCTTCTGTAATCTT953980.7624802612181061No Hit
TCCTTTCAGTGGATATTGGTATTATTGT942830.7535684864297648No Hit
AACCCAATCAAGCTTGCCATACATAAGA917240.7331153638437867No Hit
TTCGGTCTCTTACACTACGACCATTATC887540.7093772731519716No Hit
CGCAGGTAGAGTTGATATCCCACCAGAG842440.6733305428421783No Hit
AATCGTGCATGTCGTAATCACCTTCGGC726900.5809837752148277No Hit
TTCGATTTCCTTCTTCTAGTTTCACCTA699050.5587243198017957No Hit
GCACTAATCCTACCGTACCTTCCATTTG693500.5542884139654465No Hit
GTTACGAGTTGCATTGCCGCCTATGATC645910.5162515204966425No Hit
GAGGCCTTCGAGTCCGTCTGTATTTTCT619500.4951430028141227No Hit
CTCACTGTCGCGTAGCAAGGTTATTAGG584840.46744057105054315No Hit
TGTAAGCCAGGTCTCGTTTGATTCCTCT569340.4550520052012794No Hit
ACCAAACTCTTGGTGATTCTGTCAATCT516210.4125871985192547No Hit
AGTCATGTCTCTAGGAGGAAACATCAGT488490.39043164720689394No Hit
CAGGTATGTCACGCTGGGCCCTCCCTTC487950.3900000455579518No Hit
TCTATCATCGCAAGAGGGAGATCGAGAT485100.387722147966313No Hit
GCGGAAACATGTTCAGAACTACCAGCCT465670.37219248122752624No Hit
ATGACCACAGGAGGTTTTTAGAACAAAC448840.35874089650216434No Hit
ACATGCATCGTTCAGATTTTCTCGCAAG448720.35864498502462167No Hit
TCCCAGTGTGTAGGACATCTTTTTTTTA417840.3339637648036368No Hit
CTGAGGCGTCAGTCGCATTTACTTTGCA405300.323941015400426No Hit
AGTTCCCCAACCAGAGAGTTCTGAACTT398210.3182742456022789No Hit
GATTTCTAGTGTTCCAGTTCTGCCTAAC388980.31089705445462057No Hit
GACCGTGCAGTAGATAATCCAGCAACTC383640.306628993703971No Hit
CCTCCATCCTTGGTGAAACCCTTTGCGC383410.30644516337201416No Hit
GAGATGGGTAGCGATGAACCACTGCCAC378310.30236892557644995No Hit
GGGTATTGTCGGTGAAGTCTTTGTGTTC361310.28878146625790263No Hit
CATGCGGAGGATTCCTATAAACACTGAT346910.27727208895278016No Hit
TAGCACAAGAGCGACTACGCATCAGGAC346560.27699234714328064No Hit
GATCCCTGTCTGTTAGCTCCACTCCGAA329340.26322905011590503No Hit
AGCGCTGCAAGCACCCTTATTCGACACG323620.25865726968637026No Hit
GAGGGATAGTGGCAGTTCACTTTCATTG321080.2566271434117167No Hit
ATGAAAGAGAAACTACCATTTTGAGTAC318010.25417340811124967No Hit
TCGCAGGTCTGTCGTCGTCACTTAGTTT315220.25194346625838215No Hit
CCATCACCAGAATCGGAACTGTAATGTT313640.25068063180407013No Hit
TTGGGATAGCAGCACACAACTGGGACCT309990.2477633243621467No Hit
CACTAAGAGAGCCGATTATATCTGGAAT309620.24746759730639012No Hit
CAAAGAATCAAGTAAGGCTCTCTGATAA295850.23646175525836674No Hit
GTTCATTCAGTATGAAGGGACTATTCGC283550.2266308288102413No Hit
CCACGTTAGGTGCCAACTCACTAGTTGT283190.2263430943776132No Hit
TGACGCGGTGACGTCCCACCTACACAAC276480.22098004425835133No Hit
CTCTCGAAGGCTCACCTGCTATCTATAA268590.21467386460991963No Hit
CGAAGTTGTAGTGGCATTTATCCCTCTG266690.21315526621549374No Hit
GACCCTTTCTTCGCTGTATCTGGACCCT258620.206705219350748No Hit
CTGCCTACATTGTCGAACGTCTCCTCGT257130.205514318504593No Hit
CATACAGTCTCATTGTAACATTACTTCA253040.20224533564501307No Hit
AGCCACGTCTAGTTCTTAAATTGCGCAA253020.20222935039875597No Hit
AATGGAATCCGGACTGCACTCGGAATTA248580.19868062572967654No Hit
TGTCCTGTCCTGGGACAGGGTCACCGCC247070.1974737396372644No Hit
CGGACACTCGACATTGTATCACACAGCG247060.19746574701413586No Hit
TTAATCCAGCAACTTCCAGCCATGCAAT246440.19697020438016527No Hit
TATTGGGGTGTACATCATTCCCCTGGCA245640.19633079452988073No Hit
GGCTTGGGTTGGGATGACTCGCTCTATT245540.19625086829859512No Hit
TGCAGTAAGGCCATAGATGTATCCCCGG245230.19600309698160986No Hit
TTCATGTCACGTCGTGACAAAATCTGCA245150.1959391559965814No Hit
GCGTTTCTCACCCATCGCCAAACGTCCC244590.19549156910138218No Hit
TTACGCCAGCTAATCCCACGTGAACCCG241980.19340549446482874No Hit
GCACGGTAGCAGGGAGTATGTCCATCCT241650.19314173790158637No Hit
TGGTTAGAGGAGTCTGATTGTATATTTG236330.1888896623971939No Hit
GGGTCACGTTGCATTGCCGAAGTTGTTC236220.18880174354277976No Hit
TGAATCGGTTACGGAGGATAAAGCTGAG235820.18848203861763746No Hit
ACGTTCCGTCCCAAATTTGAACTGCTCG235570.18828222303942352No Hit
GCCCAGACACTATCGAACTAGTAACCGA234660.18755489433472483No Hit
GTTCATTCAATCGTCACGTGTTAAACCA234390.18733909351025377No Hit
TTGCTGCAGCGGATCAAACGTGTCAACG231000.18462959426967285No Hit
TTCTTCCCATCGTGGCTAAACGCAGAAC227210.18160039010394965No Hit
TTTCCTCAGACCAAATGTGCTGCACTCG226620.18112882533936478No Hit
AGCATCACAACCAACTGGCAAAGCTACT225800.18047343024282309No Hit
GAAACCTGTAGCGAGTTATGACCACTTT225500.18023365154896637No Hit
GTATTGGGTCCCTGTTTTCGCATGAAAG224520.17945037448236775No Hit
TTGCTGCTCGGTAAGGTTAATATATGGA224070.17909070644158268No Hit
CCGATCTGTTTGGGAGATCCGTCAGCTA222950.17819553265118426No Hit
AGAGCAGAGGCCTTCGCGTTAACATACT222930.17817954740492714No Hit
AGTCTCCCAAGGCGTAATGGGAGTTGGT220960.17660500064860138No Hit
TGTTTGTGTCACGCTGCTACTTTTAAAC220230.17602153916021668No Hit
CCATCACAGCGTGAGTGCGCCATCCGCA219210.17520629160110385No Hit
GTGGGAAAGCGAGTCACATAGGTAGGTC219030.1750624243847898No Hit
AACACACTCTCAAAGCGATAAATGAGGC218970.17501446864601847No Hit
TCGGGACCACAACATCGGTAGCAATTGT216490.17303229811013626No Hit
CTATCCAGGAATTACTTTGGACTTTGGT213890.17095421609671138No Hit
TGGGCGTAGTAAATGCCACCTGACAATA211180.16878821522887236No Hit
CGGGTCAAGGATCATAAGTTAATTACGG210740.16843653981121584No Hit
CATGGATAGATAGGGAACTGCAAGGCAC210320.16810084963981645No Hit
TTGCATTAGTAGCCAGTGTTGTTGCATG210170.1679809602928881No Hit
GGGCGTTGTAGAATGTAGGAGCGCTAGG209920.16778114471467415No Hit
GTCCTCATCAGGAAATTAATGACCTACT209850.16772519635277425No Hit
GATTTCTAGAGCCATGCGTTTTGCCTGA209070.16710177174874677No Hit
GATGAGGGTGAATTGACCTACTGCACAC208470.16662221436103333No Hit
GAACACTAGCAGGGAGGTTAATGCATAT207870.16614265697331992No Hit
CAGAGCCTCAGGAAATACTAAAGTGTTC205820.16450416923196565No Hit
TTACCGCGTGTTCGATTTATATTTCGAT205110.1639366929898381No Hit
TCCGAAAGTTCCGCAGCTCCCATCTCTT204730.16363297331095292No Hit
AGTGCCGGTGAGCGATCCTGTGGTCCCT204130.16315341592323948No Hit
CTCAGGGGTCAGTCGCTTATTCTAAGTA201740.1612431789955143No Hit
CATGCGGAGTCAACAAGGGACGTCCTCA201490.16104336341730036No Hit
GTTCATTCAGTATGAACACAAATAAACG199460.15942086092220323No Hit
CCAATTTGTGAGGAAAACATATCTGTTG198900.15897327402700404No Hit
CACGTGGTCTGGGATTCTCGGAGGTGGA198690.15880542894130434No Hit
TTCCTCTGTGGTCTTATGGAGAGTATTT198680.15879743631817578No Hit
ATGTCCCCACTCTGCTAAACAGTACACG196320.15691117725983628No Hit
TCAGGTATCGAGAAATGAACAGTGGTTC194850.15573626165993834No Hit
GGGAGTAAGCGGGTATATTCAACACTGA194460.1554245493579246No Hit
TACTTACCATCAACCATTTAAATCTCGC191700.1532185853744428No Hit
ACGTTCCCACAAACGGTAATACTAGTAC190820.15251523453912977No Hit
GCGAGAATCACGATCATTGGCTTGGCAT189590.15153214189431724No Hit
TGTCCCACATGACTCAAATCCAAGGAAG189480.1514442230399031No Hit
TGGGATTCACGACCTGTAAACCGTACTT188420.15059700498827602No Hit
TCTACATAGCAGCCCTGGTCTAGATTTT187980.1502453295706195No Hit
GCCCGAAAGTGCTACTTTCTGGTCCCTT185770.14847895985920834No Hit
TGAGGAGTCATGCCGGCCGCCAAGAGTC185760.1484709672360798No Hit
GGATGTTTCACCTCTGCCTCCTTCGTCG185210.14803137296400914No Hit
AAGTTCGAGATGACATTGCTGCCTCCGC182280.14568953438734186No Hit
TTAGTCTAGTCTACCAACCCTAGGACTT181820.14532187372342822No Hit
ACCAACACAGACTGCCTAAGTAACATCA180710.14443469255615837No Hit
TTCTAACGTCCTGTCTCAAGACTACACG179500.14346758515760294No Hit
ATAGGCTTCGTTACCCAAAGCCTTCATC178260.14247649988966185No Hit
TTCAGGATCTGACCCTACGATATTAATA178010.1422766843114479No Hit
TCCGATCAGAGGATCCCTTAATAATTGT177610.1419569793863056No Hit
ACCCTTGTCTTAATCCGTAGCTTCTTAT177310.1417172006924489No Hit
TCAGTCCCAGACGCTCATTACCTTAATC176900.14138950314417806No Hit
GTCATGAAGAAGTGTTGAGCGAGCCTCT175400.14019060967489447No Hit
AGTCAACTCTACTGCCTGCTCTTCGACG174760.13967908179466682No Hit
TGAGCGCAGTAAACGTCAGCTATTTCCT174580.13953521457835277No Hit
TATATCCGTAGAGGAATGTAGGATTAAC172390.13778483011319873No Hit
TCAGCAAGTAGCTGTTCATGAGTTTTCT172360.13776085224381304No Hit
TCAGCCTAGGGTGAAATGAATACATGTG172070.13752906617308489No Hit
TGCGGGTAGCCAGAGTTATAACAGACTG171840.13734523584112807No Hit
CGTTCTGGTAGCGCTCCCGAGACCTCTT171520.13708947190101423No Hit
TCCGAAAAGGACAACCTAAGTCACGCAA170690.136426084181344No Hit
CGACAGCCACCGGTCATCTTTTATAGCA169630.1355788661297169No Hit
GGAGGTAAGCGACTTTGCGAGCCCGAGG168590.13474763332434694No Hit
GCCCAGAAGCGCCTCAGAGGATTATTAT168310.13452383987674735No Hit
CTGCATCTCCACTTCGATCACCGTCCTT167320.13373257018702017No Hit
TTGGGTAGTACCAGAGATAGTTCATGGC164910.13180634801303787No Hit
TGATTTCTCTTCGCTGACAACATTCGTA164550.13151861358040984No Hit
AACCATGAGATGAAGGCTTTTGTGTAGT164510.1314866430878956No Hit
AGCTCAAAGTACGTCTTAACTTCTCGCA162840.13015187502542655No Hit
TTCCGTGCAAGACCGATGAGCTGATAAT162620.12997603731659826No Hit
CCTGCATTCGGTCGGTTTCTCTCAGCTT162450.12984016272341278No Hit
AAACGAAGTGCTATTGACTGTCTCATTT160950.12864126925412922No Hit
CGGGACTGTACCTGTATCCCTCTTGAGC160150.12800185940384462No Hit
TATTGCTAGGAGCTGTCAATATGGGCTA160000.12788197005691626No Hit
CAGCCAGCATTCACCCTGAATTCAGTTT159710.1276501839861881No Hit
ACGCACGCAGGCACTCAGAACTTCCGTG158940.12703475200528921No Hit
CTTACCGGTTCTCAGAAGATCGCCAGGA158040.12631541592371906No Hit
AGACAGGGTAGGCTGAGACGGGGTTCTG155820.12454105358917933No Hit
AAGGAATAGGGACTGTGAAAGTAATTCC153790.1229185510940822No Hit
ACATTTCCAAGCACAGTACTTAAGTATA153070.12234308222882609No Hit
GTGAGCCAGCTGTCCGATTTCCGTCTAA153010.12229512649005474No Hit
GCCAGCAGTGACCTGCTCCGTTGCGAAG152770.12210330353496937No Hit
CTCCAACAGGTGATCGCATAAAACCGAC151220.12086444695004299No Hit
CTCATTACAAATGCGGTGCGGATCAAAT151180.12083247645752876No Hit
ATGGTTGAGTTGGAGCTACTTCCTCTCC150640.12040087480858666No Hit
CTGGCAGCAAAGAACTTATCTTTATTTT149970.11986536905897333No Hit
GTGAGCCTCCATAAGCTGGCGCGTCGAG149340.11936183380187422No Hit
GTCGTAAAGATCCAAAGTATAATTCAAA148010.11829881492577611No Hit
CTCCAACGTTATGGTCGACCAAGATATT147760.11809899934756218No Hit
TGTTTGTTCTCCACTGGGGGTGACAAGG147140.11760345671359163No Hit
GGCTGTGAGGCAATGCGATTAGTACCGG147130.11759546409046306No Hit
GGGTCTGCATCTTCGCATGTTGGGGTTG146380.11699601735582128No Hit
CATTGTTGTGTCTTGAGAAGTTTCGTCT145950.1166523345612933No Hit
ATCGTCCAGGTGGGTTTAACATAGTTCA145270.11610883618855142No Hit
TCTCACGCAAGACGACCTCATTCTAATA145090.11596496897223739No Hit
TGGGCGTGTGCATCTATTACTGCTACCC144540.11552537470016673No Hit
AAGTACCGTAATGATGACCATTATGGAT143350.11457425254786842No Hit
ACCAAACAGCGGATCAAGCAAGTTTGTC142750.11409469516015498No Hit
GACATCATCTGTCTCGCTCCCCGACCAG142680.11403874679825508No Hit
TCCACCACAACGACTTCTTTAATGTTCC142320.11375101236562701No Hit
GTATTGGCAACTTGCAAAAACTGCTCCG142010.11350324104864175No Hit
GGTAACTGTCTGTCAACTTTCTTACTCA141980.11347926317925607No Hit
ACCTGAACATTCTTCATACGACACGTTA141290.11292777218338562No Hit
CGTAGTAAGGCCTGCTTGGTTCTTCGTT140800.11253613365008633No Hit
GCATCTCAGTGTCATCAACAGGGTTCGT139480.11148110739711675No Hit
CATCCACTCCTAAGTGACTCCTCAGAAG139340.11136921067331695No Hit
GTCAAGTTCGTGGAAGTTTAATACGTTC139210.11126530657264572No Hit
GATGACTCACATCCCTATCCAAGTACAA138820.11095359427063198No Hit
TCCGGGAAGGCACTCCGACTTATCCGCG138780.11092162377811775No Hit
TGCGGCAGTAGCGAGTTTATATTTATAT137440.10985061227889108No Hit
GAGGGATAGTGGTTAAGTTCTTCATGTG137180.10964280407754859No Hit
ACCGTTCAGGCAGCTACTTATATCTCGT137060.1095468926000059No Hit
AGGTTACCATGTGCCGGCTCTAGATAAT136530.10912328357419238No Hit
CTCAACCAGAGCATATAATTTCACTAAA136230.10888350488033566No Hit
GGGTGAAGTAGTATAGCTTTTCCATCCG134660.10762866304915215No Hit
TTCGGTCAGTGGTGGTACTACTGCGTAT134210.10726899500836708No Hit
CACTGGGGTGCTTCAAGTGGAAACAGGA132820.10615802039349763No Hit
TCACGGGCAACTCATGGCGACGGCACGC132620.10599816793092647No Hit
TTCCTCTGTAAGCGGTCTCTACTTAATG131940.10545466955818458No Hit
CGTAATGGTCCGTTTCTTAGTTTGCCCA131890.10541470644254179No Hit
GCCATGGAGCTTACGTTTAGATCTTACT131650.10522288348745641No Hit
GTCATTTAGCACGATGATAATTCACTGA131580.10516693512555651No Hit
CTCCACAAGTTTCTTCTTATTAAGGTAA131360.10499109741672824No Hit
AAAGTGATCTTTGGAGCCATTCTGGTCT130620.10439964330521503No Hit
TATCTGTTCTCCATATCAACATCGCCAC130380.10420782035012964No Hit
TTGATGGCATCCTCACCGAACACCGGCT130200.10406395313381561No Hit
AGCTCAAAGTCAATCCACGCCGTATTCT130020.10392008591750157No Hit
AGGCTGCGTCACTCAATCCCTCCTTTTC129830.103768226078059No Hit
GTGACGCCATACGCATTTCCTGATTTCG129620.1036003809923593No Hit
CCGAACGTCCATAGACCAAGCTTATTCT128870.10300093425771749No Hit
TCAGGTATCACCTCTGCACTAGCTGCGC127990.10229758342240447No Hit
AGATGCTAGATAGTCATACCGATACACG127460.1018739743965909No Hit
TTGCCTGGTGAATGTATGCAAACCGATT127310.10175408504966256No Hit
AATCGACAGGAGCAAATAACAGCATTTT127010.10151430635580584No Hit
AGGGCTCTCGAGATGGTATTTTAAGCTA126600.101186608807535No Hit
CGAATTGCAAGGACACAATCGCATGCTC125970.10068307355043588No Hit
TGCGACGTCTGAGGTTAAATTTTCAAAA125940.10065909568105022No Hit
CGAGTGCCAAGTATCCCTGATCACAGGT125920.1006431104347931No Hit
AGCCAGCGTGCGTGCTATGACTGGCTGT125910.10063511781166455No Hit
CTGCAGGGTGCATGTTACACTAATCCGA125470.10028344239400802No Hit
ATGGATCAGTTCCAGTTCTAATATACCA125160.10003567107702276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)