FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116845
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT58915.041721939321323Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC28632.450254610809192Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG27282.334716932688605Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCATCACAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACG14221.2169968762035175No Hit
GGTATCAACGCAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACG11901.0184432367666567No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG9770.8361504557319526Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA9160.7839445419145021Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8080.6915143994180324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG7900.6761093756686208Clontech Universal Primer Mix Long (96% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG7780.6658393598356798Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT7660.6555693440027387Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC7520.6435876588643075Clontech SMART CDS Primer II A (96% over 26bp)
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC7490.6410201549060721Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGACCGGCCCAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG5330.45615986991313273Clontech SMART CDS Primer II A (100% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT5120.4381873422054859Clontech SMART CDS Primer II A (96% over 26bp)
GTATCATCACAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACGA4710.4030981214429372No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC4400.37656724720783946Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACGA4290.36715306602764347No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG3900.33377551457058496Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACACAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACG2950.2524712225598014No Hit
GAGTACATGGAAGCAGTGGTATCATCACAGGGTTGGACCTGTCTCTTATAC2890.24733621464333091No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA2690.23021952158842912Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG2680.22936368693568404Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTG2570.21994950575548802Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACATGGAAGC2500.21395866318627244Clontech Universal Primer Mix Long (96% over 25bp)
ATGACCGGCCCAGTACTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA2340.200265308742351No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC2260.19341863152039027Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC2170.18571611964568444Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGCCAAGCAGTGGTATCAACGCAGAGTACAT2060.17630193846548847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAAGC1950.16688775728529248Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCATCACAGGGTTGGACC1950.16688775728529248No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC1920.16432025332705721Clontech SMART CDS Primer II A (96% over 26bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAG1670.14292438700842997Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA1600.13693354443921435Clontech Universal Primer Mix Long (96% over 25bp)
CGCAGAGTACAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACGA1570.13436604048097908No Hit
GTGATGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT1480.12666352860627325Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1470.12580769395352817Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGGGTTGGACCTGTCTCTTATAC1440.12324018999529292Clontech SMARTer II A Oligonucleotide (95% over 23bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCAC1370.11724934742607727Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT1340.11468184346784202Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAGGGTTGGACCTGTCT1310.11211433950960674Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAT1280.10954683555137147Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTATA1280.10954683555137147Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT1280.10954683555137147Clontech SMART CDS Primer II A (96% over 26bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1240.10612349694039112Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACACAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACGA1220.10441182763490094No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)