FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116845
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC33362.8550644015576188Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT27452.349266121785271Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAACCCTGTGATGATACCACTGCTTCCATGTACTCTGCGTTGATACCA27062.315888570328213No Hit
GTCCAACCCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA18871.6149599897299842Clontech Universal Primer Mix Long (95% over 23bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG14271.221276049467243Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAACCCTGTGATGATACCCTGTCTCTTATACACATCTGACGCTGCCGA14021.1998801831486157No Hit
GTCCAACCCTGTGATGATACCACTGCTTGTACTCTGCGTTGATACCACTGC12881.1023150327356754Clontech SMART CDS Primer II A (95% over 24bp)
GTCCAACCCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGA11220.9602464803799906No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT10520.9003380546878343Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC8770.7505669904574437Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC8200.7017844152509736Clontech Universal Primer Mix Long (95% over 24bp)
GTCCAACCCTGTGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA7360.629894304420386No Hit
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT5870.5023749411613676Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT5630.4818349094954854Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA5540.47413239762077963Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAACCCTGTGATGATACCTGTCTCTTATACACATCTGACGCTGCCGAC5160.4416106808164662No Hit
GTCCAACCCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGAC4600.3936839402627412No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG4130.353459711583722Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA3860.3303521759596046Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCAACCCTGTGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC3650.3123796482519577Clontech SMART CDS Primer II A (95% over 24bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG3300.28242543540587955Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG3290.2815696007531345Clontech Universal Primer Mix Long (96% over 25bp)
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC3210.2747229235311738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTG3210.2747229235311738Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG3170.2712995849201934Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGTGATGATACCACTGCTTGGCCATGTACTCTGCGTTGATAC2850.24391287603235057No Hit
GTCCAACCCTGTGATGATACCACTGCTTCCATGTACTCCTGTCTCTTATAC2840.24305704137960546No Hit
GTCCAACCCTGTGATGATACCACTGCTTCCATGTACTCTGCGTTGATACCC2800.2396337027686251No Hit
GTCCAACCCTGTGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGA2800.2396337027686251No Hit
GTCCAACCCTGTGATGATACCACTGCTTCATGTACTCTGCGTTGATACCAC2770.23706619881038982Clontech SMART CDS Primer II A (95% over 21bp)
GAGTACTGGGCCGGTCATCTGTCTCTTATACACATCTGACGCTGCCGACGA2710.2319311908939193No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTATA2650.22679618297744875Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCC1910.1634644186743121Clontech Universal Primer Mix Long (95% over 23bp)
GTCCAACCCTGTACTCTGCGCTGTCTCTTATACACATCTGACGCTGCCGAC1900.16260858402156703No Hit
GTGATGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT1840.1574735761050965Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC1750.1497710642303907Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCAC1740.14891522957764558Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG1610.13778937909195943Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1610.13778937909195943Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT1580.13522187513372416Clontech SMART CDS Primer II A (96% over 26bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1420.12152852068980273Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTATAC1420.12152852068980273Clontech Universal Primer Mix Long (95% over 23bp)
GTCCAACCCTGCGTTGATACCACTGCTTGGCCATGTACTCTGCGTTGATAC1420.12152852068980273Clontech Universal Primer Mix Long (95% over 24bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC1360.11639351277333221Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCT1340.11468184346784202Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGTGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGAC1320.11297017416235183No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA1290.11040267020411655Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTAT1280.10954683555137147Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGCCAAGCAGTGGTATCAACGCAGAGTACAT1250.10697933159313622Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1210.10355599298215584No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA1210.10355599298215584Clontech Universal Primer Mix Long (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)