FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54249
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT2030.37420044609117215Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1640.30230971999483863Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCATCACAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACG1620.29862301609246256No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1250.23041899389850506Clontech SMART CDS Primer II A (96% over 26bp)
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC1090.2009253626794964No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG1030.18986525097236814Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA900.16590167560692362No Hit
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT880.16221497170454754No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT850.15668491585098343No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC700.1290346365831628No Hit
GGTATCAACGCAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACG680.12534793268078676No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA600.11060111707128241No Hit
GGTATCAACGCAGAGTACGAAGCAGTGGTATCAACGCAGAGTACGAAGCAG570.1050710612177183Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA550.10138435731534222No Hit
CTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTG550.10138435731534222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)