FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237451
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT4028316.964763256419218Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGTACTCTGCGCTGTCTCTTATACACATCTGACGCTGCCGAC154776.517976340381805No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC151206.367629531987652Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT101284.2653010515853795Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCT100994.25308800552535Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGTACTCCTGTCTCTTATACACATCTGACGCTGCCGACGAAT73793.107588512998471No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG55442.3347974950621393Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT46631.9637735785488375Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACG29071.224252582638102No Hit
GTCCAACCCTGTGCTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT26581.1193888423295753Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT24531.033055240870748Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA20110.8469115733351302Clontech Universal Primer Mix Long (96% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG14660.617390535310443Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGTCTC14180.5971758383834981Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCCATGCTGTCTCTTAT14120.59464900126763Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13450.5664326534737694Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTATA13210.5563253050102969Clontech SMART CDS Primer II A (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG12610.5310569338516157Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC12350.5201073063495205Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT11520.4851527262466782Clontech SMART CDS Primer II A (96% over 26bp)
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11360.47841449393769664Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCAACCCTGTGCTCTGCGCTGTCTCTTATACACATCTGACGCTGCCGAC10460.44051193719967485No Hit
GTCCAACCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT10220.43040458873620246Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGC9990.42071837979204124No Hit
GTCCAACCCTGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATC9820.4135590079637483Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9460.3983979852685396No Hit
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTG9080.38239468353470824Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGTGCTCCTGTCTCTTATACACATCTGACGCTGCCGACGAAT9030.38028898593815147No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA8890.3743930326677925Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGAT8460.3562840333374043Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGTACTCTGTCTCTTATACACATCTGACGCTGCCGACGAATA7200.30322045390417396No Hit
GTCCAACCCTGTGCTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCT6030.2539471301447457Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGC4970.20930634109774227Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC4630.19498759744115626Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA4390.18488024897768382Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCT4250.17898429570732488Clontech SMART CDS Primer II A (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC4140.1743517609949Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAT3540.14908338983621885Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCT3530.1486622503169075Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGAGGTAT3500.14739883175897342Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAACCATGTACTCCTGTCTCTTATACACATCTGACGCTGCCGACGAAT3480.14655655272035073No Hit
GTCCAACCCTGCTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGA3400.1431874365658599No Hit
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACTCAGCGTT3040.12802641387065122Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAGGG3030.12760527435133986Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAACCCATGTACTCCTGTCTCTTATACACATCTGACGCTGCCGACGAA2990.12592071627409446No Hit
GTCCAACCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCT2900.12213046060029226Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA2880.12128818156166955Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGATGATACC2850.12002476300373552Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGCTGTCT2820.11876134444580146Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT2780.11707678636855605Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGTACTCTGCGTTGATACCACTGCTTCATGCTGTCTCTTATA2770.11665564684924469Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAGGGTTGGACCTGTCT2710.11412880973337657Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAGGGTTGGACCTGTCTCTTATACACATCTGACGCTGCCGACGAAT2640.1111808330981971No Hit
GTCCCTGTCTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAG2540.10696943790508358No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)