FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255364
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC8640.3383405648407763No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA7200.2819504707006469No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT6630.259629391770179No Hit
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG6500.2545386193825285No Hit
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA5930.23221754045206058No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA5770.22595197443649068No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC5550.217336821165082No Hit
CTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAA5500.2153788317852164No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA5080.19893172099434533No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCT4930.19305775285474852No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA4870.1907081655989098No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC4830.1891417740950173No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGC4730.1852257953352861No Hit
GGCCTGTCGGCCAAGGCTATAAACTCGTTGAATACATCAGTGTAGCGCGCG4710.18444259958333986No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC4570.17896022931971617No Hit
GCTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT4560.17856863144374305No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA4470.17504425055998496No Hit
AGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT4460.17465265268401184No Hit
GACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCA4420.17308626118011936No Hit
CCTAAGAGACGGGGGAAGCCCGTCTGATAGCGTGCTAAGCGCGAGCTTCGA4380.17151986967622687No Hit
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT4370.17112827180025375No Hit
GCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAGCCGACGGGTTCG4230.16564590153663006No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG4200.1644711079087107No Hit
GGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACC4180.16368791215676445No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTC3760.1472408013658934No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTAT3630.14215002897824283No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAGCGATCGTTC3630.14215002897824283No Hit
CCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTG3630.14215002897824283No Hit
TGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGA3630.14215002897824283No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGCTGT3570.13980044172240408No Hit
CCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGG3380.1323600820789148No Hit
GCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCG3370.1319684842029417No Hit
GTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGG3320.13001049482307608No Hit
CCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGG3210.12570291818737175No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACT3150.12335333093153303No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCT3140.12296173305555991No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCCT3140.12296173305555991No Hit
GCTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA3110.12178693942764054No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGT3100.12139534155166742No Hit
GTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGC3080.12061214579972118No Hit
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTACTATGTCTGG3060.11982895004777494No Hit
GTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAG3050.11943735217180183No Hit
TCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTA2960.11591297128804373No Hit
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTG2920.11434657978415125No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACCTGT2910.11395498190817813No Hit
GTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTT2880.11278018828025876No Hit
GCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC2880.11278018828025876No Hit
CTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTG2790.10925580739650069No Hit
GCCGGGGGACGGACTGGGAACGATCGCTTCGGCGGTCTTCCCCGGGCGTCG2720.10651462226468884No Hit
GCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTA2700.1057314265127426No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGTATGCTCTCACTC2680.10494823076079636No Hit
GCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATA2670.10455663288482323No Hit
GCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTT2610.1022070456289845No Hit
CCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCA2580.10103225200106514No Hit
GATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC2570.10064065412509202No Hit
CATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAA2560.1002490562491189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)