FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248608
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC72572.919053288711546Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT57672.3197161796885055Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG29611.1910316643068606Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT23580.9484811430042477Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA10340.41591581928176086Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT9630.38735680267730727Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAA8440.33949028188956104Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8040.3234006950701506Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCAACCCTGTGATGATACCACTGCTTCCATGTACTCTGCGTTGATACCA7980.320987257047239No Hit
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT7450.29966855451152014Clontech SMART CDS Primer II A (96% over 26bp)
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC6860.2759364139528897Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG6240.25099755438280347Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCAACCCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA5400.21720942206204147Clontech Universal Primer Mix Long (95% over 23bp)
GTCCAACCCTGTGATGATACCCTGTCTCTTATACACATCTGACGCTGCCGA5220.20996910799330673No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC5020.20192431458360152No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC4690.18865040545758785Clontech SMART CDS Primer II A (96% over 26bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCA4460.17939889303642684Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGA4190.16853842193332474Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGA4030.16210258720556056No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTATA4000.16089586819410479Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCA3960.15928690951216373Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA3750.15083987643197322Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC3460.13917492598790063Clontech Universal Primer Mix Long (96% over 25bp)
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC3210.1291189342257691No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC3190.12831445488479856No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT3040.12228085982751963No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA3000.12067190114557858No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA2900.11664950444072596No Hit
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAG2690.10820247136053547Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT2690.10820247136053547Clontech SMART CDS Primer II A (96% over 26bp)
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG2640.10619127300810914No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA2510.10096215729180076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)